Detoxified castor seed meal replaces soybean meal in the supplement for Holstein-Zebu crossbred steers finished on tropical pasture during the rainy season.

The objective of this study was to examine the effect of replacing soybean meal (SBM) with detoxified castor seed meal (DCM) on the intake, digestibility, feeding behavior, and performance of pasture-finished (rainy season) steers supplemented with concentrate at 0.4% of their body weight. Forty ½ Holstein + ½ Zebu steers (initial weight: 283.3 ± 36.3 kg) were allocated to four treatments in a completely randomized experimental design. Treatments consisted of diets in which DCM replaced 0, 30, 60, and 90% of SBM in the supplement dry matter (DM). The steers were finished on an Urochloa brizantha pasture and the experiment lasted 112 days. Replacing SBM with DCM did not influence (P > 0.05) the intake or apparent digestibility of DM, crude protein, or neutral detergent insoluble fiber of the animals. Grazing time increased (P < 005), whereas the intake and rumination efficiencies of the steers did not change (P > 0.05) with the substitution. The replacement of SBM with DCM in the supplement fed to the steers also did not influence (P > 0.05) their final weight, average daily gain, or feed conversion (P > 0.05). We recommend replacing up to 90% (DM basis) of SBM with DCM in the concentrate supplement of steers grazing on Urochloa brizantha pasture during rainy season while supplemented with concentrate at 0.4% of their body weight.

Creatinine as a Urinary Marker of the Purine Derivatives Excretion in Urine Spot Samples of Lambs Fed Peach Palm Meal.

The objective was to evaluate the influence of diets on lambs using different levels of peach palm meal as a replacement for maize (0, 10, 40, 60, and 85% of diet dry matter) on the endogenous creatinine clearance (CC), urine concentration ratio of purine derivatives to creatinine (PDC index), and daily creatinine excretion (DCE) as a marker to estimate purine derivatives (PD) excretion from urinary spot samples collected at different time points (4, 8, 12, 16, 20, 24 h after morning feeding) compared to 24-h total urine collection. The measured parameters were voluntary intake, urinary volume, CC, DCE, the concentration of plasma creatinine, and PD and purine derivatives' excretion (PDE). Five lambs were allocated to metabolic cages and distributed in a 5 × 5 Latin square. Urine collection was taken daily on days 16 to 19 of each experimental period. The inclusion of peach palm meal linearly reduced the intake of dry matter (g kg BW−0.75, p = 0.005), crude protein (g kg BW−0.75, p = 0.010), metabolizable energy (MJ kg BW−0.75, p = 0.010) and CC (p < 0.0001). It also quadratically affected the urinary volume (p = 0.008) and DCE (p = 0.004). There was a linear decrease for PDC index (p = 0.032) and PDE (p < 0.0001) measured in the 24-h total urine with peach palm meal levels. The different times of spot urine sampling did not affect (p > 0.05) the PDC index and PDE. Peach palm meal decreases the CC thereby compromising the use of a mean value of DCE as a PDE marker in spot urine samples. There is greater accuracy when using different values of DCE obtained for each diet as markers for the PDE in spot urine samples. Unconventional foodstuffs of low palatability affecting the voluntary intake of feed change the renal function.

Agronomic characterization of sunflower cultivars for animal feeding in tropical conditions

This study was developed to examine morpho-agronomic traits of 18 sunflower cultivars and identify superior cultivars in terms of grain yield, forage quality, or both, for animal feeding. Twenty-two morpho-agronomic traits related to plant development and architecture; earliness of maturity; grain yield (achenes); dry matter yield; and dry matter content were evaluated. Cultivars Hélio 253, Hélio 358, Embrapa 122, BRS 321, and Hélio 360 showed inflorescence at the final stage. Aguará 4 showed the lowest flowering rate, characterizing it as late-maturing. For grain yield, cultivars Charrua, Olisun 3, BRS 321, Paraíso 103CL, Paraíso 65, Aguará 6, and CF 101 are recommended, as they showed the highest achene yields (average: 1,541.67 to 2,148.81 kg.ha−1, respectively). Cultivars Charrua, Hélio 251, Olisun 3, Hélio 360, Paraíso 55, and Paraíso 103CL exhibited higher dry matter yields (9,550.93 to 11,789.91 kg ha−1) and were thus indicated for forage production. Cultivars Charrua, Olisun 3, BRS 321, Paraíso 103CL, Paraíso 65, Aguará 6, and CF 101 are recommended for grain yield, for the diet of monogastric animals; Charrua, Hélio 251, Olisun 3, Hélio 360, Paraíso 55, and Paraíso 103CL for forage yield, for ruminant feeding; and Charrua, Olisun 3, and Paraíso 103CL for both purposes.

Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.

The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot. Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement. The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW). The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits. The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets. There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan. Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.

Influence of body condition score at calving on the metabolic status and production performance of Murrah buffaloes (Bubalus bubalis) during the transition period.

OBJECTIVE: The purpose of this study was to evaluate the influence of body condition score (BCS) at calving on the metabolic status of female Murrah buffaloes in the transition period. METHODS: Thirty-seven pregnant buffaloes (multiparous) were selected and monitored during the transition period based on their body condition score and on the estimated calving date. Two groups were formed: i) buffaloes with a BCS>3.5 (n = 17); this group was classified and named 'high BCS at calving' (HBCS); and ii) buffaloes with a BCS≤3.5 (n = 20); this group was classified and named 'low BCS at calving' (LBCS). All animals were monitored during the last 30 days of pregnancy and the first 70 days post-calving and kept in the same environment and under the same feeding and management conditions. Mean values for BCS at calving were 2.98±0.9 (mean±standard error of the mean [SEM]) and 4.21±0.9 (mean±SEM) for the HBCS and LBCS groups, respectively. RESULTS: The HBCS group showed higher milk fat content (p = 0.007) and milk fat yield (p = 0.027) and a higher concentration of milk urea nitrogen (p = 0.001) than LBCS buffaloes, which in turn had a lower urine pH value (p = 0.033) than HBCS buffaloes in the pre-calving period (7.86 for HBCS vs 7.76 for LBCS). The HBCS animals had a higher concentration of erythrocytes (p = 0.001) and hematocrit (p = 0.012) post-calving and a higher hemoglobin concentration (p = 0.004) pre-calving. CONCLUSION: Buffaloes during the transition period exhibited some variations in the oxidative stress related to their metabolic status. After calving, buffaloes with a high BCS at calving and greater lipid mobilization have a more marked alteration in oxidative status, but improved production performance.

Biomass turnover in brachiaria cultivars in a tropical environment / Fluxo de biomassa em cultivares de brachiaria em ambiente tropical

The experiment was conducted to evaluate the morphogenetic traits of three Brachiaria cultivars (common, Llanero and Tupi) subjected to continuous grazing with variable stocking rate in the four seasons of the year. A randomized block design with five replicates was adopted. Tissue turnover evaluations were carried out in the summer, fall, winter and spring, with measurements taken every two days. Stem length and the length of each green leaf were measured. With these determinations, the following variables were calculated: stem elongation rate (cm/tiller/day), leaf elongation rate (cm/leaf/day), number of live leaves per tiller and phyllochron (days/leaf). The highest leaf appearance rate was obtained by Tupi grass in the summer, and the lowest by Llanero grass in the winter. The lowest phyllochron, 5.90 days/leaf, was obtained by Tupi grass in the winter, whereas in commercial cultivars Llanero and common the average phyllochron was 9.04 and 10.04 days/leaf, respectively. Because of the effect of the transition from the dry season to the beginning of rains in the summer, this was the period of greatest variations in results among the different cultivars. Llanero grass stood out for the highest values for plant, tiller and leaf lengths, resulting from greater stem and leaf elongation rates. Tupi grass had the worst performance of all cultivars.

O experimento foi realizado com o objetivo de avaliar as características morfogenéticas de três cultivares de Brachiaria humidicola (common, Llanero e Tupi), submetido a pastejo contínuo, em quatro estações do ano. Foi utilizado delineamento em blocos casualizados, com cinco repetições. As avaliações do volume de tecido vegetal foram realizadas no verão, outono, inverno e primavera, e as medidas foram tomadas a cada dois dias. Determinaram-se o comprimento da haste e o comprimento de cada lâmina de folha verde. Com essas determinações, foram calculados: taxa de alongamento (cm / perfilho / dia), taxa de alongamento foliar (cm / folha / dia), número de folhas vivas por perfilho e filocromo (dia / folha). A maior taxa de aparecimento de folhas foi obtida na grama Tupi no período de verão, e o menor aparecimento ocorreu no cultivar Llanero, durante o inverno. O menor filocromo (5,90 dias / folha) foi verificado no cultivar Tupi, no inverno, enquanto no cultivares Llanero e common o filocromo médio foi de 9,04 e 10,04 dias / folha, respectivamente. Devido ao efeito da transição, a partir da estação seca para o início das chuvas no verão, foi constatado que as maiores variações nos resultados dos diferentes cultivares, ocorreram nesta época. O cultivar, Llanero se destacou para os maiores valores da planta, resultante das maiores taxas de alongamento de caule e folhas. O cultivar Tupi teve o menor desempenho em comparação com os outros.

Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu(2+) ions.

The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu(2+) cryogel system was studied by batch equilibrium measurements. The adsorptive equilibrium data were obtained at 17 and 27°C, pH 5.0 and 6.5, and at ionic strength of 50 and 200mmolL(-1) NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu(2+) cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27°C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27mgg(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.

Degradação ruminal da silagem de capim-elefante aditivado com cana-de-açúcar e farelo de cacau / Ruminal degradation of elephant grass sillage added with sugarcane and cocoa meal

Objetivou-se avaliar a degradação ruminal da matéria seca (MS), da proteína bruta (PB) e da fibra em detergente neutro (FDN) das silagens de capim-elefante; capim-elefante ensilado com 30 por cento de cana-de-açúcar; 15 por cento de farelo de cacau; e 45 por cento de cana mais 15 por cento de farelo de cacau. Amostras de cada silagem foram incubadas no rúmen de três novilhos mestiços holandês x zebu durante os períodos de 0, 12, 24, 48, 72 e 96 horas. O delineamento experimental utilizado foi o de parcelas subdivididas e os coeficientes não lineares a, b e c foram estimados por meio de procedimentos iterativos de Gauss-Newton. Para o capim-elefante ensilado com farelo de cacau, foram observados maiores valores da fração a da MS e da PB, próximos a 60 por cento, além de maiores taxas da degradação da fração b e da degradabilidade efetiva (DE) da PB para as taxas de passagem 2, 5 e 8 por cento h-1. As silagens que apresentaram as maiores frações não-degradáveis i da FDN foram as silagens de capim-elefante sem aditivo e com 30 por cento de cana, enquanto a silagem aditivada com 45 por cento de cana e 15 por cento de farelo de cacau destacou-se pela maior degradação potencial da MS, da PB e da FDN, em todos os períodos estudados, e maiores DE da MS e da PB para as taxas de passagem 2, 5 e 8 por cento h-1. A inclusão associada da cana-de-açúcar com o farelo de cacau, no momento da ensilagem do capim-elefante, apresenta-se como alternativa para aumentar a degradabilidade da forragem em estádio de crescimento avançado.

The objective was to evaluate the dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation of silages of elephant grass; elephant grass ensiled with 30 percent of sugarcane; 15 percent of cocoa meal; and 45 percent of sugarcane plus 15 percent of cocoa meal. Samples of each silage were incubated in the rumen of three Holstein x Zebu crossbred steers during the periods of 0, 12, 24, 48, 72 and 96 hours. The experimental design used was the one of subdivided parcels and the non linear coefficients a, b and c were estimated through Gauss-Newton interactive procedures. For the elephant grass ensiled with cocoa meal, larger values of DM and CP fraction a were observed, next to 60 percent, besides the greatest b fraction degradation rates and effective degradability (ED) of CP to passage rates of 2, 5 and 8 percent h-1. The silages that showed the largest non-degradable i fractions of NDF were the elephant grass silages without additive and with 30 percent of sugarcane, while the silage addicted with 45 percent of sugarcane and 15 percent of cocoa meal showed up by the largest DM, CP and NDF potential degradation in all of the studied periods, and larger DM and CP ED for passage rates of 2, 5 and 8 percent h-1. The associated inclusion of sugarcane with cocoa meal at the moment of the elephant grass ensilage comes as an alternative to increase the degradability of forage in advanced growth stage.